Aminoacylation of tRNAs Encoded by Chlorella Virus CVK2
Identifieur interne : 003968 ( Main/Exploration ); précédent : 003967; suivant : 003969Aminoacylation of tRNAs Encoded by Chlorella Virus CVK2
Auteurs : Kensho Nishida [Japon] ; Takeru Kawasaki [Japon] ; Makoto Fujie [Japon] ; Shoji Usami [Japon] ; Takashi Yamada [Japon]Source :
- Virology [ 0042-6822 ] ; 1999.
English descriptors
- Teeft :
- Accession, Acid sequence similarity, Acidic, Acidic conditions, Aminoacylated, Aminoacylation, Bacteriophage, Blot analysis, Chlorella, Chlorella cells, Chlorella strain, Chlorella virus, Chlorella virus cvk2, Chlorella virus genome, Chlorella viruses, Codon, Codon usage, Cven5, Cvk2, Cvk2 genome, Cvk2 trnas, Ddbj accession, Escherichia coli, Etten, Eukaryotic, Genbank accession, Gene, Genome, Host cells, Hybridization, Hybridized, Insertion, Intron, Kutish, Large precursor, Major capsid protein, Nishida, Northern blot analysis, Nucleic acids, Nucleotide sequence, Polymerase, Size markers, Trna, Trna genes, Trna sequences, Uninfected, Viral, Viral gene products, Viral trnas, Virology, Yamada, Yeast.
Abstract
Abstract: Viruses that infect certain strains of the unicellular green alga, Chlorella, have a large, linear dsDNA genome that is 330–380 kb in size; this genomic size is the largest known among viruses and is equivalent to ∼60% of the smallest prokaryotic genome of Mycoplasma genitalium (580 kb). Besides many putative protein-coding genes, a cluster of 10–15 tRNA genes is present in these viral genomes. Some of these tRNA genes contain peculiar insertions. In infected host cells, the viral tRNAs of CVK2, a Chlorella virus isolate, have been demonstrated to be cotranscribed as a large precursor, ∼1.0 kb in size, that is precisely processed into individual mature tRNA species. Acidic Northern blot analysis of eight of these tRNAs has revealed that they are actually aminoacylated in vivo, indicating their involvement in viral protein synthesis. They may help the virus reach maximal replication potential by overcoming codon usage barriers that exist between the virus and its host. These results provide evidence that some components of the host protein synthesis machinery can be replaced by viral gene products. This is the first report of tRNA aminoacylation encoded by viruses of eukaryotes.
Url:
DOI: 10.1006/viro.1999.9949
Affiliations:
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Besides many putative protein-coding genes, a cluster of 10–15 tRNA genes is present in these viral genomes. Some of these tRNA genes contain peculiar insertions. In infected host cells, the viral tRNAs of CVK2, a Chlorella virus isolate, have been demonstrated to be cotranscribed as a large precursor, ∼1.0 kb in size, that is precisely processed into individual mature tRNA species. Acidic Northern blot analysis of eight of these tRNAs has revealed that they are actually aminoacylated in vivo, indicating their involvement in viral protein synthesis. They may help the virus reach maximal replication potential by overcoming codon usage barriers that exist between the virus and its host. These results provide evidence that some components of the host protein synthesis machinery can be replaced by viral gene products. 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